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primary anti alpha-smooth muscle actin (α-sma)  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc primary anti alpha-smooth muscle actin (α-sma)
    Primary Anti Alpha Smooth Muscle Actin (α Sma), supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 790 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+%CE%B1+sma+primary+antibody/alpha-Smooth+Muscle+Actin+Antibody/pmc13009532-51-0-15
    Average 96 stars, based on 790 article reviews
    primary anti alpha-smooth muscle actin (α-sma) - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: ERK1/2 Signaling Pathway Activated by EGF Promotes Proliferation, Transdifferentiation, and Migration of Cultured Primary Newborn Rat Lung Fibroblasts
    Article Snippet: .. Membranes were blocked for 1 h with 5% nonfat milk followed by incubation with anti-p-ERK1/2, anti-ERK, anti-type Ι collagen (Col- Ι ), or anti- α -SMA primary antibody (#4370 for p-ERK1/2, #4696 for ERK1/2, #91144S for Col- Ι , #56856 for α -SMA; all 1 : 1000; Cell Signaling Technology, Boston, MA, USA) overnight at 4°C, and then with HRP-labeled secondary antibody (ZB2305, 1 : 2000; Beijing Zhongshan Goldenbridge Biotechnology, Beijing, China) at 37°C for 2 h. The blots were developed using electrochemiluminescence after washing three times with Tris-buffered saline containing Tween 20. ..

    Article Title: miRNA‐130b‐5p promotes hepatic stellate cell activation and the development of liver fibrosis by suppressing SIRT4 expression
    Article Snippet: .. HSC‐T6 cells were incubated with anti‐α‐SMA primary antibody (Cell Signaling Technology), and then, the cells were treated with secondary goat anti‐rabbit Texas Red–conjugated IgG (Sigma) and DAPI. .. After washing the slides twice with pre‐cooled PBS, we used confocal microscopy (ZEISS, Oberkochen) to obtain fluorescence images of the cells according to the manufacturer's instructions.

    Article Title: Chaihu shugan powder ameliorated chronic pancreatitis by reprogramming SMS2-mediated sphingolipid metabolism in M1 macrophages
    Article Snippet: Background: Chronic pancreatitis (CP) lacks targeted therapies.. Its pathogenesis involves macrophage polarization-activated pancreatic stellate cells (PSCs), with sphingolipid metabolism playing an emerging role.. The traditional formula Chaihu shugan powder (CSP) shows clinical potential for CP treatment.

    Electrochemiluminescence:

    Article Title: ERK1/2 Signaling Pathway Activated by EGF Promotes Proliferation, Transdifferentiation, and Migration of Cultured Primary Newborn Rat Lung Fibroblasts
    Article Snippet: .. Membranes were blocked for 1 h with 5% nonfat milk followed by incubation with anti-p-ERK1/2, anti-ERK, anti-type Ι collagen (Col- Ι ), or anti- α -SMA primary antibody (#4370 for p-ERK1/2, #4696 for ERK1/2, #91144S for Col- Ι , #56856 for α -SMA; all 1 : 1000; Cell Signaling Technology, Boston, MA, USA) overnight at 4°C, and then with HRP-labeled secondary antibody (ZB2305, 1 : 2000; Beijing Zhongshan Goldenbridge Biotechnology, Beijing, China) at 37°C for 2 h. The blots were developed using electrochemiluminescence after washing three times with Tris-buffered saline containing Tween 20. ..

    Saline:

    Article Title: ERK1/2 Signaling Pathway Activated by EGF Promotes Proliferation, Transdifferentiation, and Migration of Cultured Primary Newborn Rat Lung Fibroblasts
    Article Snippet: .. Membranes were blocked for 1 h with 5% nonfat milk followed by incubation with anti-p-ERK1/2, anti-ERK, anti-type Ι collagen (Col- Ι ), or anti- α -SMA primary antibody (#4370 for p-ERK1/2, #4696 for ERK1/2, #91144S for Col- Ι , #56856 for α -SMA; all 1 : 1000; Cell Signaling Technology, Boston, MA, USA) overnight at 4°C, and then with HRP-labeled secondary antibody (ZB2305, 1 : 2000; Beijing Zhongshan Goldenbridge Biotechnology, Beijing, China) at 37°C for 2 h. The blots were developed using electrochemiluminescence after washing three times with Tris-buffered saline containing Tween 20. ..



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    Glutamate metabolic and glutamate receptor (GRIN2D) is increased in keloids and fibroblasts. (A) FPKM values of glutamate receptor genes (GRIN2D, GRIA1, GRIA2, GRIK1, GRIK2, GRIK5, GRIN1, GRIM1, GRIMX, GRIN1B) in keloid compared to normal skin controls. (B) Dot plot of single-cell RNA-seq data depicting the distribution of GRIN2D and related glutamate receptor genes across major skin cell populations. (C) Expression levels of GRIN2D across different cell types in CTRL and KD skin, showing preferential upregulation in fibroblasts from KD lesions. (D) Representative immunohistochemical staining for GRIN2D in CTRL and KD tissues, demonstrating stronger GRIN2D signal in keloid dermis. (E) Immunofluorescence <t>of</t> <t>α-SMA</t> and GRIN2D in keloid and normal skin tissues. (F,G) Heatmap (F) and bar graph (G) of targeted metabolomics for glutamic acid and glutamine in individual CTRL and KD samples, illustrating a shift in glutamine–glutamate metabolism in KD. p-values were determined by one-way ANOVA: *p < 0.05, **p < 0.01, ***p < 0.001.
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    Glutamate metabolic and glutamate receptor (GRIN2D) is increased in keloids and fibroblasts. (A) FPKM values of glutamate receptor genes (GRIN2D, GRIA1, GRIA2, GRIK1, GRIK2, GRIK5, GRIN1, GRIM1, GRIMX, GRIN1B) in keloid compared to normal skin controls. (B) Dot plot of single-cell RNA-seq data depicting the distribution of GRIN2D and related glutamate receptor genes across major skin cell populations. (C) Expression levels of GRIN2D across different cell types in CTRL and KD skin, showing preferential upregulation in fibroblasts from KD lesions. (D) Representative immunohistochemical staining for GRIN2D in CTRL and KD tissues, demonstrating stronger GRIN2D signal in keloid dermis. (E) Immunofluorescence <t>of</t> <t>α-SMA</t> and GRIN2D in keloid and normal skin tissues. (F,G) Heatmap (F) and bar graph (G) of targeted metabolomics for glutamic acid and glutamine in individual CTRL and KD samples, illustrating a shift in glutamine–glutamate metabolism in KD. p-values were determined by one-way ANOVA: *p < 0.05, **p < 0.01, ***p < 0.001.
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    Image Search Results


    Glutamate metabolic and glutamate receptor (GRIN2D) is increased in keloids and fibroblasts. (A) FPKM values of glutamate receptor genes (GRIN2D, GRIA1, GRIA2, GRIK1, GRIK2, GRIK5, GRIN1, GRIM1, GRIMX, GRIN1B) in keloid compared to normal skin controls. (B) Dot plot of single-cell RNA-seq data depicting the distribution of GRIN2D and related glutamate receptor genes across major skin cell populations. (C) Expression levels of GRIN2D across different cell types in CTRL and KD skin, showing preferential upregulation in fibroblasts from KD lesions. (D) Representative immunohistochemical staining for GRIN2D in CTRL and KD tissues, demonstrating stronger GRIN2D signal in keloid dermis. (E) Immunofluorescence of α-SMA and GRIN2D in keloid and normal skin tissues. (F,G) Heatmap (F) and bar graph (G) of targeted metabolomics for glutamic acid and glutamine in individual CTRL and KD samples, illustrating a shift in glutamine–glutamate metabolism in KD. p-values were determined by one-way ANOVA: *p < 0.05, **p < 0.01, ***p < 0.001.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Glutamate enhances the production of inflammatory cytokines IL-6 and IL-11, as well as chemokines CXCL2, CXCL3, and CXCL8 in keloid fibroblasts

    doi: 10.3389/fmolb.2025.1720876

    Figure Lengend Snippet: Glutamate metabolic and glutamate receptor (GRIN2D) is increased in keloids and fibroblasts. (A) FPKM values of glutamate receptor genes (GRIN2D, GRIA1, GRIA2, GRIK1, GRIK2, GRIK5, GRIN1, GRIM1, GRIMX, GRIN1B) in keloid compared to normal skin controls. (B) Dot plot of single-cell RNA-seq data depicting the distribution of GRIN2D and related glutamate receptor genes across major skin cell populations. (C) Expression levels of GRIN2D across different cell types in CTRL and KD skin, showing preferential upregulation in fibroblasts from KD lesions. (D) Representative immunohistochemical staining for GRIN2D in CTRL and KD tissues, demonstrating stronger GRIN2D signal in keloid dermis. (E) Immunofluorescence of α-SMA and GRIN2D in keloid and normal skin tissues. (F,G) Heatmap (F) and bar graph (G) of targeted metabolomics for glutamic acid and glutamine in individual CTRL and KD samples, illustrating a shift in glutamine–glutamate metabolism in KD. p-values were determined by one-way ANOVA: *p < 0.05, **p < 0.01, ***p < 0.001.

    Article Snippet: Primary antibodies α-SMA (Proteintech, 14395-1-AP) and GRIN2D (Proteintech, 27232-1-AP) were diluted in blocking solution as per the manufacturer’s instructions and incubated overnight at 4 °C or for 1–2 h at room temperature.

    Techniques: RNA Sequencing, Expressing, Immunohistochemical staining, Staining, Immunofluorescence